Our lab develops tools to empower the neuroscience community. We believe that tools should be as simple as possible, while addressing broad conceptual questions.
For example, DART (Drugs Acutely Restricted by Tethering) makes it possible to deliver traditional drugs to defined cell types in a behaving animal. There are two steps:
(1) first, a virus is used to select the cells of interest.
(2) weeks later, any drug from of our catalog can be delivered exclusively to these cells, with no effect on neighboring cells.
As with traditional pharmacology, DART involves no overexpression of the receptor of interest, and drug effects have acute onset. Unlike traditional pharmacology, DART is cell type-specific. This makes it possible to map "drug x cell-type" effects on behavior, without caveats of overexpression or compensatory artifacts.
We keep making DART better. Our priorities are to expand the available drug catalog, and to make the tool even easier to use via whole-brain non-invasive dosing. If you have an interest in DART, we collaborate extensively, and are eager to help.
Our lab develops tools to empower the neuroscience community. We think tools should be simple, while addressing broad conceptual questions.
For example, DART (drug acutely restricted by tethering) makes it possible to deliver pharmaceuticals to defined cells of a behaving animal―in two steps:
first, a virus is used to select the cells of interest.
weeks later, any drug in our catalog can be delivered to these cells, with no effect on neighboring cells.
Like traditional pharmacology, DART has acute onset and involves no receptor overexpression. DART is also cell type-specific. These features are unique, making it possible to map "drug x cell-type" effects on behavior, without overexpression or compensatory artifacts.
We keep making DART better, and are expanding the drug catalog. If you have an interest in DART, we collaborate extensively, and are eager to help.
GRADUATES & ALUMNI
May 2023, Ankit Choudhury ― Tech II → Univ. of Wisconsin Medical School
Mar. 2023, Victoria Goldenshtein ― PhD in Biomedical Engineering → Postdoc & Entrepreneur
Feb. 2023, Kyle Jensen ― Postdoctoral Associate → Sophion & ExstoBio
Oct. 2022, Isaac Weaver ― PhD in Biomedical Engineering
May 2022, Austin Li ― BS in Biomedical Engineering → Case Western Medical School
Mar. 2021, Elizabeth Kahuno ― Associate in Research → U. Mass. Med. School / Melikian Lab
Aug. 2018, Jane Tandler ― Masters in Neurobiology → Development Director, StepUp Durham
Shields BC*, Yan H*, Lim SSX, Burwell SC, Fleming EA, Cammarata CM, Kahuno EW, Vagadia PP, Loughran MH, Zhiquan L, McDonnell ME, Scalabrino ML, Thapa M, Hawley TM, Reitz AB, Schiltz GE, Hull C, Field GD, Glickfeld LL, Tadross MR. "Thousandfold Cell-Specific Pharmacology of Neurotransmission." biorxiv doi:10.1101/2022.10.18.512779 (2022).
Shields BC*, Kahuno EW*, Kim C*, Apostolides PF*, Brown J, Lindo S, Mensh BD, Dudman JT, Lavis LD, Tadross MR‡. "Deconstructing behavioral neuropharmacology with cellular specificity." Science 356, no. 6333 (2017).
Click here to see our full list of publications
Pratt School of Engineering
Bryan Research Building
311 Research Drive, Room 331
Durham, NC, 27710